Product Info Summary
SKU: | FEK0351 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Mouse Fibronectin PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0351
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Fibronectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Fibronectin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0351)
Clonality of Antibodies
See Datasheet for details
Immunogen
Target protein isolated from mouse plasma
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0351 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0351 is reactive to FN1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
FEK0351 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fibronectin
Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK0351-CAP | 96-well plate precoated with anti-Mouse Fibronectin antibody | 1 |
EK0351-ST | lyophilized recombinant Mouse Fibronectin standard | 10 ng/tube |
FEK0351-DA | Biotinylated anti-Mouse Fibronectin detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.048 | 0.092 | 0.179 | 0.264 | 0.366 | 0.741 | 1.258 | 1.990 |
Data Example Images
Click image to see more details
Mouse Fibronectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 206 | 1683 | 4323 | 219 | 1843 | 3947 |
Standard deviation | 13.39 | 126.22 | 293.96 | 15.54 | 160.34 | 335.49 |
CV (%) | 6.5% | 7.5% | 6.8% | 7.1% | 8.7% | 8.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Fibronectin in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 206 | 229 | 235 | 215 | 221 | 11.4 | 5.1% |
Sample 2 | 1683 | 1597 | 1749 | 1766 | 1698 | 66.42 | 3.9% |
Sample 3 | 4323 | 4412 | 4796 | 4012 | 4385 | 279.56 | 6.3% |
Protein Target Info & Infographic
Gene/Protein Information For FN1 (Source: Uniprot.Org, NCBI)
Uniprot ID
P02751
Gene ID
2335
Gene Name
FN1
Full Name
Fibronectin
Weight
272.32kDa
Alternative Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FN1, check out the FN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Fibronectin PicoKine® Quick ELISA Kit (FEK0351)
Hello CJ!
FEK0351 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Damage-associated molecular patterns (DAMPs) released after burn are associated with inflammation and monocyte activation
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